Methylcobalamin

6125.

IHS

Vitamin (Henatopoietic)

TP (Technical Package)

[13422-55-4]

Cobinamide Co-methyl deriv. di hydrogen phosphate (ester) inner salt 3prime-ester with 5,6-di methyl-1-alpha-D-ribofuranosyl-1 H- benzimidazole

methyl-5,6-di methylbenz imid azolyl cobalamin; mecobalamin; methyl vita min B 12 ; MeCbl

Algobaz (Labaz) ; Hitocoba min-M (Hishiyama) ; Methycobal (Eisai) ; Methyl cobaz (Labaz)

C 63 H 91 CoN 13 O 14 P

1344.40

C 56.28%, H 6.82%, Co 4.38%, N 13.54%, O 16.66%, P 2.30% .

One of the biologically active forms of vitamin B 12 , q.v. ; differing only by the substitution of a methyl for the cyano group. Coenzyme required in the biosynthesis of methio nine. Identification as requirement for methionine biosynthesis by E. coli : R. L. Kisliuk, D. D. Woods, Biochem. J. 75, 467 (1960); J. R. Guest et al., Nature  195, 340 (1962). Prepn: O. Müller, G. Müller, Biochem. Z. 336, 299 (1962); D. Dolphin, Methods Enzymol. 18, Pt. C, 34 (1971); D. Autissier et al., Bull. Soc. Chim. France 1980, part 2, 192. Interrelationship with folate metabolism: B. Shane, E. L. R. Stokstad, Ann. Rev. Nutr. 5, 115-141 (1985). Discussion of mechanism of action: W. S. Beck, Am. J. Hematol. 34, 83-89 (1990). Review: "Vitamin B 12 " in Vita mins , W. Fried rich, Ed. (de Gruyter, Berlin, 1988) pp 837-928.

Bright red crystals from water/acetone. uv max (pH 7): 522, 342 , 266 nm (epsi 9357, 14416, 19897); (0.1 N HCl): 462, 304 , 264 nm (epsi 9599, 22855, 24737)

342; 266; 304;